Detection of transgenic events in maize using immunochromatographic strip test and conventional PCR
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چکیده
منابع مشابه
Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip
BACKGROUND Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic t...
متن کاملdetection of staphylococcus enterotoxin b (seb) using an immunochromatographic test strip
results in total, 80 food samples suspected of seb contamination were assessed using the two methods. fifty-four samples were contaminated based on the pcr technique and twenty-six of those were confirmed using the strip assay. conclusions the sensitivity of the sandwich method was approximately 10 ng/ml and that of the competitive method was approximately 250 ng/ml. in the lfd, a highly specif...
متن کاملA fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection.
A fluorescent immunochromatographic strip test (ICST) based on the use of Quantum Dots (QD) was developed and applied to detect fumonisins in maize samples. A limit of detection for fumonisin B1 of 2.8 µg L(-1) was achieved, with an analytical working range of 3-350 µg L(-1), corresponding to 30-3500 µg kg(-1) in maize flour samples, according with the extraction procedure. The time required to...
متن کاملDevelopment of an Immunochromatographic Strip Test for Rapid Detection of Ciprofloxacin in Milk Samples
A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-bu...
متن کاملDevelopment of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit
A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits dete...
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ژورنال
عنوان ژورنال: Ciência e Agrotecnologia
سال: 2013
ISSN: 1413-7054
DOI: 10.1590/s1413-70542013000500003